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Replacement of the dryer completely depends upon how often it is used to dry hydrolysis samples. The filter should be changed when the filter bags do not dry sufficiently after hydrolysis at 110°C for three hours (adding pH paper in the desiccant...
When using the ANKOM Rapid Dryer to dry acid hydrolysis samples, the airflow through the special hydrolysis filter ( Part #: RD2 ) will eventually become too limited and the filter will need to be changed. To check the air filter for proper airflow...
When samples are dried for three hours after hydrolysis, they should be placed in a desiccant pouch ( Part #: X45 ) with a strip of pH paper. When the bags are fully dry, the pH paper will NOT show the presence of acid.
This can occur with samples that are very low in fat. Moisture left in low-fat samples after extraction is the issue. As AOCS Procedure AM 5-04 indicates, when the Filter Bags are removed from the Extractor, they should be placed in an oven at 100...
You can see unexpectedly high values when hydrolyzing fat samples if the samples are not adequately dried after the hydrolysis step. Possible reasons for insufficient drying include: (1) Dryer is not working properly - Filter needs to be replaced -...
Possible Causes: (1) The vent tube or drain tube is blocked. The blockage is caused by water trapped in the line. It is very important that the vent and drain tubes run downhill and are not submerged in water. If the vent tube is blocked, a vacuum is...
Possible Causes: (1) The dial on the heat sealer may not be turned up high enough. To seal the XT4 Bag, the dial must be set at approximately "6." (2) The heating element is broken. On the sides of the Teflon cover are two shiny metal strips that...
When the lid is raised, some residual acid may flow onto the hinges and the handle. Over time, corrosion will occur if this happens. When lifting the lid after a run, it is recommended that the lid be wiped off with a paper towel to prevent dripping...
If a Mojonnier solvent (pet ether, ethyl ether, ethanol) is utilized, a blank value of 0.0030g is normal. If pet ether or hexanes are used, a blank value of 0.0010g is normal. During the hydrolysis phase, fat migration may occur with samples that...
To determine how much DE to use with various sample types, refer to the Hydrolysis Procedure subsection titled Sample Categorization of the HCl Operator's Manual.
To perform an extraction of oilseeds, refer to Oilseed Extractions (FOSFA Equivalent).
Research with high fat samples has given us the following understandings: Some samples with fats that have low melting point triacylglycerides or emulsifiers may encounter fat migration during hydrolysis. To improve fat retention, increase the level...
Some samples with large amounts of carbohydrates (starches and cellulose) may bind small quantities of solvent and require additional post drying. To remove the bound solvent, dry at 100-105° C for one to three hours.
The Overtemp message happens when the ANKOM HCl Instrument is heating but there is no solution in the vessel. The quartz heaters will heat the vessel and the ETS (Emergency Temperature Switches) will trip. Possible Causes: The instrument was not...
View the HCL Periodic Maintenance video .
To understand how to mix DE with various sample types, view the How to mix DE with samples video.
In developing our hydrolysis process, we did extensive research into acid and base hydrolysis. We found that the acid hydrolysis did well with a variety of samples, including those normally hydrolyzed in a base solution, i.e., dairy products. As a...
High Fat Samples are defined as any meat sample with a fat content >20% and any plant sample with a fat content >40%. See XT Service Procedure High Fat Sample Handling (XTS014).
The enzyme tubing should be changed annually under normal use. Heavy use may necessitate a shorter timeframe between tubing changes. The Enzyme Tubing Replacement Kit ( Part #: TDF67 ) is available for purchase by contacting ANKOM Technology at (315)...
This issue may occur if the instrument is not fully purged of the residual enzyme left in the supply lines, especially Trizma. ANKOM recommends flushing the enzyme supply lines with alcohol weekly when enzymes are being used. Further, we recommend...
The enzyme tubing may be clogged. Look at the enzyme tubing from the reservoirs to the Enzyme Pinch Valve and beyond to the manifold. If there is any discoloration or gelled material in the tubing, replace the tubing. Contact ANKOM Technology at...
Refer to TDF Service Procedure E13 Diagnosis (TS001) to diagnose tubing pressure failure.
The following are two possible reasons why enzymes may not be drawn from the reservoir. (1) The silicone tubes may be worn or flattened from use. To inspect the tubing, refer to TDF Service Procedure Tube Replacement (Pinch Valves) (TS005) and...
Do NOT run this instrument without an adequate DRY Nitrogen Supply. The instrument is powered by pneumatics that are not compatible with moisture, commonly found in compressed air. Nitrogen is also recommended as an extra precaution when handling...
The MoistureStop Desiccant Pouch is a small, airtight zipper bag, utilized for desiccating all of the Filter Bags that are used with ANKOM instrumentation. A single pouch can hold a full run of F57, F58 or XT4 bags at one time. When folded, 6 IDF or...
This problem could point to one of four possible issues with the instrument. Investigate in the order below. (1) There could be a hole in the suspect bag position’s fluid line after or within the Pump causing a leak in the back of the instrument. To...
The touchscreen display will give you the option to Retry , Override , or Abort the run. Retry will simply try the delivery again. Override will ignore the fault for the remainder of the run. Abort will stop the run entirely. To analyze the problem...
We have found that it is best to slowly ramp up the digestion temperatures so that foaming in the digestion tubes is minimized. As a result, we would suggest the following times and temperatures: 15 minutes at 150°C, followed by 15 minutes at 250°C...
Order the Fill Nozzle Replacement Kit ( Part #: TDF79 ) by contacting ANKOM Technology at (315) 986-8090 or by ordering online. Refer to the TDF Service Procedure Fill Nozzle Installation Procedure (TS014).
If you do the de-fat procedure using ANKOM XT4 filter bags, after you complete the Kjeldahl digestion, there will be a white substance on top of the resulting liquid. This substance is the PTFE coating on the XT4 filter bags. This substance will NOT...
An E11 fault is the result of the high-pressure sensor reading input pressure below 35 psi. N2 input pressure is always monitored and the fault could occur at any point during a run. The fault will interrupt the run and wait for the operator to...
The Silicone Pump Tubing ( Part #: TDF68 ) should be changed when the instrument prompts the user with an E13 warning for weak pump tubes. The pump tubes typically last 6 months under normal use. Heavy usage may necessitate a more abbreviated...
Yes. Please refer to TDF Service Procedure Return Shipping Instructions (TS019) for details.
Static electricity during the weighting process: The largest contributor to poor results is the effect of static electricity on the weighing process. Because plastic gloves can contribute to static electricity, only use anti-static gloves. To...
To properly prepare the IDF & SDF bags for Kjeldahl and Ashing procedures, refer to Section 17 of the TDF Operator’s Manual (Ash Determination) or view the following video: Kjeldahl & Ashing Bag Preparation .
For instruments with version 3.78 or newer software perform the following diagnostics routine: (1) Run a Line Charge two times with “All” selected with the flush tube assembly installed using deionized water. This will prime the lines in the...
Refer to TDF Service Procedure Tube Replacement Enzymes (TS004) and review the steps related to relaxing or pre-stressing the replacement silicone tubing by rolling them with a rod or dowel.
Some variability in filter times is normal. Default filter time settings encompass much of this variability. Default settings for IDF/SDF filtration are as follows: IDF after the release of bar B (five minutes), IDF after water rinses (one minute)...
If the sample is mixing poorly at one or a few positions, the mixing pads may not be making good contact with the bag and paddle behind it. Refer to TDF Service Procedure Mixing Pad Adjustment (TS010). If the sample is mixing poorly in general, the...
This fault indicates that one or more of the Paddle Temperature Sensors is reporting erroneously low data of < 5°C. The fault message on the Touch Screen Display will indicate which station is having the problem and the heater to this station will be...
Follow the steps in this instructional video to perform a pH adjustment: TDF Manually Measuring pH in the IDF Digestion . Or, reference section 13.22 of the TDF Operator’s Manual. Note: Measure within the appropriate pH range for your specific...
Refer to the TDF Operator's Manual, sections "IDF/SDF Analysis" and "TDF Analysis."
The touchscreen display will give you the option to Retry , Override , or Abort the run. Retry will simply try the delivery again. Override will ignore the fault for the remainder of the run. Abor t will stop the run entirely. Possible causes include...
Cause: This fault occurs when the temperature sensor to the in-line heater reports that the in-line heater is not reaching the required 80˚C within the 90 seconds allotted during an EtOH95 delivery. The fault message on the touchscreen display will...
An E10 fault is the result of one or more of the six pressure sensors reading a pressure 20 psi above the baseline pressure during the buffer delivery to the IDF bags or during the EtOH 95 delivery to the SDF bags. This fault will abort the run. The...
AOAC 991.43 calls for filtration via a fritted crucible of 40-60 microns with Diatomaceous earth, or equivalent. Particle retention per the method is accomplished by adding diatomaceous earth contained in a fritted crucible. The fine filtration and...
Utilize a transfer pipette to handle this and follow the procedure below: (1) Weigh the pipette and tare the weight. (2) Fill the pipette with a liquid sample. (3) Place the pipette on the balance and record the weight. (4) Pipette out the liquid...
To correct this problem, try one or both of the following procedures: (1) During the de-fat procedure, do two 10-minute soaks in petroleum ether. This is particularly helpful with high fat (> than 20%) samples. (2) It is possible you have a sample...
In the past, we recommended customers to de-fat in XT4 bags. When the defatting process was completed, the XT4 bags were cut up and placed in the IDF bag. If the XT4 bag is cut into very small pieces, the sharp edges of the cut pieces can penetrate...
IMPORTANT: This message can be falsely declared if your instrument has been idle for a period of time. To reduce the chance of false declarations always run a line charge or two at the beginning of a routine, especially after a period of idle time (e...