What should my hydrolysis blank bag value be? Why would my blank bag value be higher than expected?

If a Mojonnier solvent (pet ether, ethyl ether, ethanol) is utilized, a blank value of 0.0030g is normal. If pet ether or hexanes are used, a blank value of 0.0010g is normal.

During the hydrolysis phase, fat migration may occur with samples that have fat with low melting points such as triacylglycerides or emulsifiers. Fat loss can be detected by placing an empty bag next to suspect samples in the holder and performing the hydrolysis. If the empty bag weight is higher than usual during the extraction, this indicates fat loss from the neighboring sample during the hydrolysis. We recommend a greater amount of diatomaceous earth and a reduced sample size with any sample showing this indication of fat loss.