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View the HCL Periodic Maintenance video .
To understand how to mix DE with various sample types, view the How to mix DE with samples video.
Refer to the TDF Operator's Manual, sections "IDF/SDF Analysis" and "TDF Analysis."
Yes, you can wash RF bottles in a laboratory dishwasher if the water temperature does NOT exceed 180°F (82°C) . Exposing the bottles to higher temperatures can damage the coating.
The touchscreen display will give you the option to Retry , Override , or Abort the run. Retry will simply try the delivery again. Override will ignore the fault for the remainder of the run. Abor t will stop the run entirely. Possible causes include...
"----" indicates there has been an electrical malfunction. Reset the Controller. Refer to A2000 Service Procedure Controller (Temp) Menu Setup (AS015). If the "----" still shows, you will need to replace the Ascon Controller ( Part #: A09 ). Possible...
Cause: This fault occurs when the temperature sensor to the in-line heater reports that the in-line heater is not reaching the required 80˚C within the 90 seconds allotted during an EtOH95 delivery. The fault message on the touchscreen display will...
An E10 fault is the result of one or more of the six pressure sensors reading a pressure 20 psi above the baseline pressure during the buffer delivery to the IDF bags or during the EtOH 95 delivery to the SDF bags. This fault will abort the run. The...
AOAC 991.43 calls for filtration via a fritted crucible of 40-60 microns with Diatomaceous earth, or equivalent. Particle retention per the method is accomplished by adding diatomaceous earth contained in a fritted crucible. The fine filtration and...
Possible Causes: Faulty A2000 Temp Controller ( Part #: A11 ). Refer to Service Procedure Controller Temp Test (AS014). Faulty Splat Controller ( Part #: A09 ) Poor Connection between the Temp Controller and the Splat Controller (AS020).
In developing our hydrolysis process, we did extensive research into acid and base hydrolysis. We found that the acid hydrolysis did well with a variety of samples, including those normally hydrolyzed in a base solution, i.e., dairy products. As a...
Utilize a transfer pipette to handle this and follow the procedure below: (1) Weigh the pipette and tare the weight. (2) Fill the pipette with a liquid sample. (3) Place the pipette on the balance and record the weight. (4) Pipette out the liquid...
To correct this problem, try one or both of the following procedures: (1) During the de-fat procedure, do two 10-minute soaks in petroleum ether. This is particularly helpful with high fat (> than 20%) samples. (2) It is possible you have a sample...
In the past, we recommended customers to de-fat in XT4 bags. When the defatting process was completed, the XT4 bags were cut up and placed in the IDF bag. If the XT4 bag is cut into very small pieces, the sharp edges of the cut pieces can penetrate...
Refer to Service Procedure Agitation and System Check (DES031).
Refer to XT15 Service Procedure Solvent Drain & Flush (X5S019) linked below.
IMPORTANT: This message can be falsely declared if your instrument has been idle for a period of time. To reduce the chance of false declarations always run a line charge or two at the beginning of a routine, especially after a period of idle time (e...
It is possible to follow an NDF run with an ADF run using the same sample and Filter Bag. However, samples that are high in pectin, tannins, glucans, and fructans can produce lower-than-expected ADF values when NDF was run first. This is because...
"----" indicates there has been an electrical malfunction. You will need to reset the Controller. Refer to DELTA Service Procedure Controller Menu Setup (DES015). If the "----" still shows, you will need to replace the Ascon Controller ( Part #: A09...
F57 The porosity of the ANKOM F57 Filter Bag is 25 microns. The design of the filter bag provides a three-dimensional filter matrix similar to the filtering effect of a glass crucible. The F57 Filter Bag provides equal filtration without the manual...
When the instrument is turned on, the drain valve opens to vent the vessel. If the drain open/close switch is not sensed by the computer, this fault occurs. Possible Causes: The wires on the drain valve have dislodged. Connections may have become...
Refer to XT15 Service Procedure Changing the Solvent (X5S007).
There are three primary reasons for high Crude Fat results. (1) The bag is not completely sealed; therefore the sample gets lost during extraction. (2) A bag picks up surface fat from another sample. (3) Incomplete drying in the pre-extraction phase...
High Fat Samples are defined as any meat sample with a fat content >20% and any plant sample with a fat content >40%. See XT Service Procedure High Fat Sample Handling (XTS014).
One ANKOM FLEX run includes four assays and will be completed in approximately two hours. Therefore, in an eight-to-ten-hour workday, the instrument can be run four times, resulting in sixteen assays per day, and eighty assays per five-day workweek...
The FLEX can process up to 10g of sample for vitamin and cholesterol analysis. The sample size maximum is dependent on analyte content, analyte homogeneity within the sample, and sample composition. Samples that typically require 10g sample sizes are...
The SPE column contains silica-based sorbent.
No. The digestion vessels must not be left assembled for extended periods of time (e.g. overnight). If digestion vessels are left assembled for extended periods of time, the O-rings in the vessel bottom assemblies will deform and future integrity...
Our grinding recommendation for samples to be extracted in our Fiber Analyzer is: 1 mm with a Wiley Mill 2 mm with a Cyclone Mill (such as UDY or Retsch)
The FLEX can run one to four samples during a run. This is configured through the user interface at the start of the method. Each time the instrument is run, all four vessels, SPE columns, and round bottom flasks must be installed. Only the...
Nitrogen is used to create an oxygen-free environment, expedite solvent evaporation, and for the general function of mechanical components.
Possible Causes: (1) The bag suspender is worn or not properly functioning. Refer to DELTA Service Procedure Bag Suspender Check (DES006). (2) The level sensor is not detecting the solution. Refer to DELTA Service Procedure Level Sensor (Analog)...
A "Low Pressure" error occurs when the vessel does not have at least 3 psi. Possible Causes: The lid was not tight. You do not have to over-tighten the lid, but it needs to be snug. Usually finger tight plus 1/4 turn is adequate. Check the lid O-Ring...
Possible Causes: The solution temperature has exceeded the maximum limit. Perform the boil test detailed in the DELTA Service Procedure Boil Test (DES013).
There are several possible reasons for a cumulative pressure drop or negative pressure readings. Refer to RF Service Procedure Curve Identification Chart (RFS010) for curve illustrations. Possible causes include: A leak in the system. First, be sure...
Refer to RF Service Procedure Module Testing (RFS001). First, check the pressure within the module. If the pressure is approaching 10 psi, stop using this module and unscrew the module top immediately to avoid over-pressurizing the bottle. If the...
The pressure sensors are accurate to +/-0.04 psi. Fluctuations in cumulative pressure beyond this correspond to either a leak or a fluctuation in temperature when a module is attached to a sealed bottle. If you check the "valve open" check box, leave...
Refer to RF Service Procedure Gas Sampling for Further Testing (RFS008).
Refer to RF Service Procedure CO2 Absorption (RFS009).
To ensure that the controller settings are correct, refer to A200 Service Procedure Controller Reset Procedure (FS002) for instruments purchased pre-9/03, or, see A200 Service Procedure Controller Menu Setup (FS001) for instruments purchased post...
When the pressure in the vessel is too high, the pressure switch will turn off the heater. Possible Causes: There is too much solution in the vessel. Make sure two liters or less of solution is used in the vessel at a time. The motor is not turning...
Refer to Service Procedure Agitation System Check (DES031).
First, make sure that your Daisy is not placed under an Air Conditioner or fan. Check your Daisy instrument bulbs, controller, and temperature probe to ensure they are functioning properly. With further questions or concerns, please contact ANKOM...
The drain valve has been told to open, but after 30 seconds the drain-open switch has failed to open the drain. Possible Causes: (1) The wires on the drain valve have become disconnected. Unplug the instrument, check the drain valve, and reattach any...
Yes. A check sample, Infant Formula, ( Part #: FLEX65 ) can be purchased on the ANKOM website.
The SPE columns and vessel filters can only be purchased through ANKOM.
In the event of a Drain Time-Out Fault (MF7) or an Abort during a Drain Module, users must flush the lines beneath the vessels to clear the residue prior to starting a new run. For more information, refer to Section 17 of the FLEX Operator’s Manual...
The preprogrammed vitamin methods are based on a combination of the following official methods: Vitamin & Cholesterol methods based on: AOAC 2011.07, EN-12823-1, EN-12822 Total Fat method: The test portion is treated under heated hydrochloric acid...
Check to ensure the procedures sent with your instrument were followed. Some possible variables that may affect your results are: General Problems: Neutral Detergent chemicals can come out of solution. Heat and stir to bring back into solution. The...
Carry-over is extremely low (~0.08%) and therefore undetectable for most food and feed samples. When running concentrates, such as vitamin supplements, use a wash bottle to rinse the round bottom flask adapters with hexane prior to the next run to...