Can I do sequential analysis using FBT?

It is possible to follow an NDF run with an ADF run using the same sample and Filter Bag. However, samples that are high in pectin, tannins, glucans, and fructans can produce lower-than-expected ADF values when NDF was run first. This is because these components are solubilized and removed by NDF solution but not by ADF solution. Therefore, these components are not present in the sample when an ADF analysis follows an NDF analysis. When you test samples high in these components, ADF results will be artificially lower than if you were to run an ADF analysis without a pre-run of NDF.  

Caution when drying the bags: When running a Lignin procedure or a sequential NDF/ADF or NDF/ADF/Lignin with the F57 Filter Bag, it is important not to dry the bags overnight after the NDF or ADF procedure. A drying timeframe of 2-4 hours at 102°C +/- 2°C is sufficient to thoroughly dry the bags after each procedure. Extended drying times or too high of a temperature can compromise the bag's filtration media.